!autogenerated-by:     DAG-Edit version 1.311
!saved-by:             luisa
!date:                 Tue Oct 28 15:21:02 CET 2003
!version: $Revision: 1.1 $
!type: % ISA Is a
!Protein:protein interactions ontology
!Luisa Montecchi Palazzi; contact: luisa@cbm.bio.uniroma2.it
!
!Notes: 
!Each of the top level terms in this file is the root term for one controlled vocabulary
!in the PSI Molecular Interaction XML schema. 
!To facilitate handling, they all have been combined in this file, though they
!are essentially independent ontologies describing different aspects of 
!molecular interactions.
!
!The PSI MI schema defines short labels for controlled vocabulary terms.
!Where possible, short labels are preceding the full term in this file,
!separated by "colon blank". Example:
!nmr: nuclear magnetic resonance ; MI:0077 ; 
!
!The last accession number used in this file is stored in a separate file,
!psi-mi.lastac.
!It MUST be updated when this file is updated.
!
!
$protein:protein interactions ; MI:0000
 %feature detection ; MI:0003
  %alanine scanning ; MI:0005
  %correlated mutations ; MI:0026 % sequence based prediction ; MI:0101
  %de novo protein sequencing by mass spectrometry ; MI:0032 % protein sequence identification ; MI:0093
  %deletion analysis ; MI:0033
  %docking ; MI:0035 % structure based prediction ; MI:0105
  %domain profile pairs ; MI:0037 % experimental knowledge based ; MI:0046 % sequence based prediction ; MI:0101
  %electron resonance ; MI:0043 % biophysical ; MI:0013
   %endor: electron nuclear double resonance ; MI:0041 ; synonym:ENDOR
   %epr: electron paramagnetic resonance ; MI:0042 ; synonym:EPR ; synonym:ESR
  %full identification by sequencing ; MI:0056 % nucleotide sequence identification ; MI:0078
  %mass detection of residue modification ; MI:0068
  %mobility shift ; MI:0070
  %mutation analysis ; MI:0074
  %neural network on interface properties ; MI:0076
  %nmr: nuclear magnetic resonance ; MI:0077 ; synonym:NMR % biophysical ; MI:0013
  %protein staining ; MI:0094
  %surface patches ; MI:0106
  %western blot ; MI:0113 ; synonym:Immuno blot % participant detection ; MI:0002
   %monoclonal antibody ; MI:0072
   %polyclonal antibody ; MI:0086
  %x-ray: x-ray crystallography ; MI:0114 ; synonym:X-ray % biophysical ; MI:0013
 %feature type ; MI:0116
  %binding site ; MI:0117
  %hotspot ; MI:0119
  %mutation ; MI:0118
  %ptm: post translation modification ; MI:0120
   %acetylation ; MI:0121
    %N-acetyl-L-alanine ; MI:0122 ; synonym:AAC ; synonym:[A:ac]
    %N-acetyl-L-arginine ; MI:0123 ; synonym:RAC ; synonym:[R:ac]
    %N-acetyl-L-asparagine ; MI:0124 ; synonym:NAC ; synonym:[N:ac]
    %N-acetyl-L-aspartic acid ; MI:0125 ; synonym:DAC ; synonym:[D:ac]
    %N-acetyl-L-cysteine ; MI:0126 ; synonym:CAC ; synonym:[C:ac]
    %N-acetyl-L-glutamic acid ; MI:0128 ; synonym:EAC ; synonym:[E:ac]
    %N-acetyl-L-glutamine ; MI:0127 ; synonym:QAC ; synonym:[Q:ac]
    %N-acetyl-L-histidine ; MI:0130 ; synonym:HAC ; synonym:[H:ac]
    %N-acetyl-L-isoleucine ; MI:0131 ; synonym:IAC ; synonym:[I:ac]
    %N-acetyl-L-leucine ; MI:0132 ; synonym:LAC ; synonym:[L:ac]
    %N-acetyl-L-methionine ; MI:0135 ; synonym:MAC ; synonym:[M:ac]
    %N-acetyl-L-phenylalanine ; MI:0136 ; synonym:FAC ; synonym:[F:ac]
    %N-acetyl-L-proline ; MI:0137 ; synonym:PAC ; synonym:[P:ac]
    %N-acetyl-L-serine ; MI:0138 ; synonym:SAC ; synonym:[S:ac]
    %N-acetyl-L-threonine ; MI:0139 ; synonym:TAC ; synonym:[T:ac]
    %N-acetyl-L-tryptophan ; MI:0140 ; synonym:WAC ; synonym:[W:ac]
    %N-acetyl-L-tyrosine ; MI:0141 ; synonym:YAC ; synonym:[Y:ac]
    %N-acetyl-L-valine ; MI:0142 ; synonym:VAC ; synonym:[V:ac]
    %N-acetylglycine ; MI:0129 ; synonym:GAC ; synonym:[G:ac]
    %N2-acetyl-L-lysine ; MI:0133 ; synonym:KAC ; synonym:[K:ac]
    %N6-acetyl-L-lysine ; MI:0134 ; synonym:KA6 ; synonym:[K:N6ac]
   %amidation ; MI:0143
    %L-alanine amide ; MI:0144 ; synonym:AAM ; synonym:[A:am]
    %L-arginine amide ; MI:0145 ; synonym:RAM ; synonym:[R:am]
   %formylation ; MI:0146
    %N-formyl-L-methionine ; MI:0147 ; synonym:MFM ; synonym:[M:form]
   %hydroxylation ; MI:0148
    %4-hydroxy-L-proline ; MI:0149 ; synonym:HYP ; synonym:[P:hy_g]
   %lipid modification ; MI:0150
    %N-myristoyl-glycine ; MI:0155 ; synonym:GMY ; synonym:[G:myr]
    %N-palmitoyl-L-cysteine ; MI:0153 ; synonym:CPN ; synonym:[C:palm_n]
    %N6-myristoyl-L-lysine ; MI:0156 ; synonym:KMY ; synonym:[K:myr]
    %S-farnesyl-L-cysteine ; MI:0151 ; synonym:CFN ; synonym:[C:farn]
    %S-geranylgeranyl-L-cysteine ; MI:0152 ; synonym:CGR ; synonym:[C:ger]
    %S-palmitoyl-L-cysteine ; MI:0154 ; synonym:CPS ; synonym:[C:palm_s]
   %methylation ; MI:0157
    %3'-methyl-L-histidine ; MI:0164 ; synonym:MHS ; synonym:[H:meth_n4]
    %L-beta-methylthioaspartic acid ; MI:0161 ; synonym:DM2 ; synonym:[D:meth_b]
    %L-glutamic acid 5-methyl ester ; MI:0163 ; synonym:EM5 ; synonym:[E:meth_o5]
    %N\,N\,N-trimethyl-L-alanine ; MI:0159 ; synonym:AM3 ; synonym:[A:meth_n3]
    %N-methyl-L-alanine ; MI:0158 ; synonym:AMT ; synonym:[A:meth_n]
    %N-methyl-L-methionine ; MI:0168 ; synonym:MMT ; synonym:[M:meth]
    %N-methyl-L-phenylalanine ; MI:0169 ; synonym:FMT ; synonym:[F:meth]
    %N5-methyl-L-glutamine ; MI:0162 ; synonym:QM5 ; synonym:[Q:meth_n5]
    %N6\,N6\,N6-trimethyl-L-lysine ; MI:0167 ; synonym:M3L ; synonym:[K:meth_3]
    %N6\,N6-dimethyl-L-lysine ; MI:0166 ; synonym:MLY ; synonym:[K:meth_2]
    %N6-methyl-L-lysine ; MI:0165 ; synonym:MLZ ; synonym:[K:meth_1]
    %omega-N\,omega-N-dimethyl-L-arginine ; MI:0160 ; synonym:RM2 ; synonym:[R:meth_n7]
   %other modification ; MI:0179
    %2'-[3-carboxamido-3-(trimethylammonio)propyl]-L-histidine (diphthamide) ; MI:0185 ; synonym:HDP ; synonym:[H:diph]
    %2-pyrrolidone-5-carboxylic acid ; MI:0183 ; synonym:PCA ; synonym:[E:pyro]
    %L-3-oxoalanine ; MI:0182 ; synonym:SOX ; synonym:[S:oxal]
    %L-glutamyl 5-glycerylphosphorylethanolamine ; MI:0184 ; synonym:EGE ; synonym:[E:gpe]
    %L-selenocysteine ; MI:0180 ; synonym:CSE ; synonym:[C:sel]
    %L-selenomethionine ; MI:0181 ; synonym:MSE ; synonym:[M:sel]
    %N6-(4-amino-2-hydroxybutyl)-L-lysine (hypusine) ; MI:0187 ; synonym:KHY ; synonym:[K:hypu]
    %N6-biotinyl-L-lysine ; MI:0186 ; synonym:KBT ; synonym:[K:biotin]
    %N6-retinal-L-lysine ; MI:0188 ; synonym:KRT ; synonym:[K:retin]
    %ubiquitinated ; MI:0189 ; synonym:KUB ; synonym:[K:ub]
   %phosphorylation ; MI:0170
    %1'-phospho-L-histidine ; MI:0174 ; synonym:HPE ; synonym:[H:po_e]
    %3'-phospho-L-histidine ; MI:0175 ; synonym:HPD ; synonym:[H:po_d]
    %L-aspartic 4-phosphoric anhydride ; MI:0172 ; synonym:DPO ; synonym:[D:po]
    %O-phospho-L-serine ; MI:0176 ; synonym:SPO ; synonym:[S:po]
    %O-phospho-L-threonine ; MI:0177 ; synonym:TPO ; synonym:[T:po]
    %O4'-phospho-L-tyrosine ; MI:0178 ; synonym:YPO ; synonym:[Y:po]
    %omega-N-phospho-L-arginine ; MI:0171 ; synonym:RPO ; synonym:[R:po]
    %S-phospho-L-cysteine ; MI:0173 ; synonym:CPO ; synonym:[C:po]
 %interaction detection ; MI:0001
  %experimental ; MI:0045
   %affinity chromatography technologies ; MI:0004 ; synonym:Affinity purification
    %coip: coimmunoprecipitation ; MI:0019 % copurification ; MI:0025
     %anti bait coip: anti bait coimmunoprecipitation ; MI:0006
     %anti tag coip: anti tag coimmunoprecipitation ; MI:0007
      %flag tag coip: flag tag coimmunoprecipitation ; MI:0050
      %ha tag coip: ha tag coimmunoprecipitation ; MI:0060
      %his tag coip: his tag coimmunoprecipitation ; MI:0062
      %myc tag coip : myc tag coimmunoprecipitation ; MI:0075
      %tap tag coip: tap tag coimmunoprecipitation ; MI:0109 ; synonym:TAP ; synonym:tandem affinity purification
    %pull down ; MI:0096
     %gst pull down ; MI:0059
     %his pull down ; MI:0061
   %array technologies ; MI:0008
    %peptide array ; MI:0081
    %pisa: protein in situ array ; MI:0092 ; synonym:PISA
    %protein array ; MI:0089
    %seldi chip: proteinchip(r) on a surface-enhanced laser desorption/ionization ; MI:0095 ; synonym:SELDI ProteinChip
   %biophysical ; MI:0013
    %cd: circular dichroism ; MI:0016 ; synonym:CD
    %electron microscopy ; MI:0040 ; synonym:Electron cryomicroscopy ; synonym:Electron crystallography
    %electron resonance ; MI:0043 % feature detection ; MI:0003
     %endor: electron nuclear double resonance ; MI:0041 ; synonym:ENDOR
     %epr: electron paramagnetic resonance ; MI:0042 ; synonym:EPR ; synonym:ESR
    %fluorescence technologies ; MI:0051
     %bret: bioluminescence resonance energy transfer ; MI:0012 ; synonym:BRET ; synonym:LRET % colocalization/visualisation technologies ; MI:0023
     %classical fluorescence spectroscopy ; MI:0017
     %facs: fluorescence-activated cell sorting ; MI:0054 ; synonym:FACS ; synonym:Flow cytometry
      %bacterial display ; MI:0009 % display technologies ; MI:0034
      %yeast display ; MI:0115 % display technologies ; MI:0034
     %fcs: fluorescence correlation spectroscopy ; MI:0052 ; synonym:FCS
     %fps: fluorescence polarization spectroscopy ; MI:0053 ; synonym:FPS ; synonym:Fluorescence anisotropy
     %fret: fluorescent resonance energy transfer ; MI:0055 ; synonym:FRET ; synonym:FRET analysis ; synonym:RET % colocalization/visualisation technologies ; MI:0023
    %itc: isothermal titration calorimetry ; MI:0065 ; synonym:ITC
    %light scattering ; MI:0067
     %dynamic light scattering ; MI:0038
     %static light scattering ; MI:0104
    %mass spectrometry studies of complexes ; MI:0069
    %nmr: nuclear magnetic resonance ; MI:0077 ; synonym:NMR % feature detection ; MI:0003
    %spa: scintillation proximity assay ; MI:0099 ; synonym:RIA Radio Immuno Assay ; synonym:SPA
    %surface plasmon resonance ; MI:0107 ; synonym:BIAcore(r) ; synonym:Optical biosensor
    %x-ray: x-ray crystallography ; MI:0114 ; synonym:X-ray % feature detection ; MI:0003
   %colocalization/visualisation technologies ; MI:0023
    %bret: bioluminescence resonance energy transfer ; MI:0012 ; synonym:BRET ; synonym:LRET % fluorescence technologies ; MI:0051
    %colocalization by electron microscopy ; MI:0020
    %colocalization by fluorescent probes cloning ; MI:0021
    %colocalization by immunostaining ; MI:0022 ; synonym:Immunofluorescence Staining ; synonym:Immunostaining
    %fret: fluorescent resonance energy transfer ; MI:0055 ; synonym:FRET ; synonym:FRET analysis ; synonym:RET % fluorescence technologies ; MI:0051
   %copurification ; MI:0025
    %coip: coimmunoprecipitation ; MI:0019 % affinity chromatography technologies ; MI:0004
     %anti bait coip: anti bait coimmunoprecipitation ; MI:0006
     %anti tag coip: anti tag coimmunoprecipitation ; MI:0007
      %flag tag coip: flag tag coimmunoprecipitation ; MI:0050
      %ha tag coip: ha tag coimmunoprecipitation ; MI:0060
      %his tag coip: his tag coimmunoprecipitation ; MI:0062
      %myc tag coip : myc tag coimmunoprecipitation ; MI:0075
      %tap tag coip: tap tag coimmunoprecipitation ; MI:0109 ; synonym:TAP ; synonym:tandem affinity purification
    %cosedimentation ; MI:0027
     %cosedimentation in solution ; MI:0028
     %cosedimentation through density gradients ; MI:0029
    %molecular sieving ; MI:0071 ; synonym:Gel Filtration ; synonym:Siezing column
   %display technologies ; MI:0034
    %bacterial display ; MI:0009 % facs: fluorescence-activated cell sorting ; MI:0054
    %mrna display ; MI:0073
    %phage display ; MI:0084
     %filamentous phage display ; MI:0048
     %lambda phage display ; MI:0066
     %t7 phage display ; MI:0108
    %ribosome display ; MI:0098
    %yeast display ; MI:0115 % facs: fluorescence-activated cell sorting ; MI:0054
   %other biochemical technologies ; MI:0079
    %cross-linking studies ; MI:0030
     %cross-linking with a bifunctional reagent ; MI:0031 ; synonym:Label transfer techniques ; synonym:Photoaffinity labelling
    %elisa: enzyme-linked immunosorbent assay ; MI:0044 ; synonym:ELISA
    %far western blotting ; MI:0047 ; synonym:Affinity blotting
    %filter binding ; MI:0049 ; synonym:Filter overlay assay
   %pca: protein complementation assay ; MI:0090 ; synonym:PCA
    %adenilate cyclase complementation ; MI:0014
    %beta galactosidase complementation ; MI:0010
    %beta lactamase complementation ; MI:0011
    %dehydrofolate reductase reconstruction ; MI:0111
    %reverse rrs: reverse ras recruitment system ; MI:0097 ; synonym:reverse RRS
    %two hybrid ; MI:0018 ; synonym:2H
     %two hybrid array ; MI:0397
     %two hybrid pooling approach ; MI:0398
      %two hybrid fragment pooling approach ; MI:0399
    %ubiquitin reconstruction ; MI:0112
  %in silico ; MI:0063
   %experimental knowledge based ; MI:0046
    %domain profile pairs ; MI:0037 % feature detection ; MI:0003 % sequence based prediction ; MI:0101
    %interologs mapping ; MI:0064 ; synonym:Homology based interaction prediction % sequence based prediction ; MI:0101
    %text mining ; MI:0110
     %confirmational text mining ; MI:0024
     %predictive text mining ; MI:0087
   %genome based prediction ; MI:0058
    %domain fusion ; MI:0036 ; synonym:Rosetta Stone % sequence based prediction ; MI:0101
    %gene neighbourhoods ; MI:0057
    %phylogenetic profile ; MI:0085
    %sequence based phylogenetic profile ; MI:0100 % sequence based prediction ; MI:0101
   %sequence based prediction ; MI:0101
    %correlated mutations ; MI:0026 % feature detection ; MI:0003
    %domain fusion ; MI:0036 ; synonym:Rosetta Stone % genome based prediction ; MI:0058
    %domain profile pairs ; MI:0037 % experimental knowledge based ; MI:0046 % feature detection ; MI:0003
    %interologs mapping ; MI:0064 ; synonym:Homology based interaction prediction % experimental knowledge based ; MI:0046
    %sequence based phylogenetic profile ; MI:0100 % genome based prediction ; MI:0058
   %structure based prediction ; MI:0105
    %docking ; MI:0035 % feature detection ; MI:0003
 %interaction type ; MI:0190
  %aggregation ; MI:0191
 %participant detection ; MI:0002
  %nucleotide sequence identification ; MI:0078 ; synonym:sequence cloning
   %full identification by sequencing ; MI:0056 % feature detection ; MI:0003
   %partial dna sequence identification by hybridization ; MI:0080
    %primer specific pcr ; MI:0088
    %southern blot ; MI:0103
  %peptide massfingerprinting ; MI:0082
  %protein sequence identification ; MI:0093
   %de novo protein sequencing by mass spectrometry ; MI:0032 % feature detection ; MI:0003
   %edman degradation ; MI:0039
   %peptide synthesis ; MI:0083
   %sequence tag identification ; MI:0102
  %undetermined participant ; MI:0396
  %western blot ; MI:0113 ; synonym:Immuno blot % feature detection ; MI:0003
   %monoclonal antibody ; MI:0072
   %polyclonal antibody ; MI:0086