High-throughput, Scalable, Quantitative, Cellular Phenotyping using X-Ray Tomographic Microscopy

Kevin Mader [1,2,3], Leah Rae Donahue [4], Ralph Müller [5], and Marco Stampanoni [1,2]
8 April 2014

  1. Swiss Light Source, Paul Scherrer Institut, 5232 Villigen, Switzerland
  2. Institute for Biomedical Engineering, University and ETH Zurich, 8092 Zurich, Switzerland
  3. 4Quant, Zurich, Switzerland
  4. The Jackson Laboratory, Bar Harbor, ME, United States
  5. Institute for Biomechanics, ETH Zurich, 8093 Zurich, Switzerland

Paul Scherrer Institut ETH Zurich 4Quant

Outline

  • Introduction
  • Motivation

    • Big Data
    • Scalability
  • Scalability

    • Imaging
    • Numerical Analysis
  • Results

  • Performance

  • Beyond

Introduction

  • A phenotype is a very general term for any measurable characteristic resulting from a combination of genetic background and environmental conditions
    • All fields of genetics are ultimately dependant on phenotypes since they are the observable differences
  • Structural phenotypes are related to position, size, shape, and organization of different cells, tissues, and organs